Fig. 3: SPINDOC is associated with DDR as revealed by RNA sequencing and GESA analysis. | Nature Communications

Fig. 3: SPINDOC is associated with DDR as revealed by RNA sequencing and GESA analysis.

From: SPINDOC binds PARP1 to facilitate PARylation

Fig. 3: SPINDOC is associated with DDR as revealed by RNA sequencing and GESA analysis.

a Heatmap of differentially expressed genes in two independent pairs of HEK293T WT and KO cell lines that were used from RNA-sequencing analysis. b The MA plot shows the overlap of differentially expressed genes from the RNA-seq dataset. Red dots are upregulated genes (Up), blue dots are down-regulated genes (Down), gray dots are nonsignificant genes (NS), and marked genes are DNA-damage and repair-associated genes. c Differentially expressed gene sets (SPINDOC KO vs WT) were enriched for hallmark of “G2M Checkpoint” and “UV response UP” as established by GESA analysis. d Upregulated, downregulated and nondifferentiated genes were validated by RT-qPCR. Graphs represent mean ± SD, n = 3 biologically independent samples. Statistical analysis was performed using one-tailed Student’s t-test with CHEK2P2: HEK293T, P = 0.0032, Hela, P = 0.0367; PPP2R2C: HEK293T, P = 0.0049, Hela, P = 0.0011; CCND2: Hela, P = 0.0432; ***P < 0.001, ****P < 0.0001, NS: nonsignificant.

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