Fig. 1: Experimental system.
From: Dynamic mechanochemical feedback between curved membranes and BAR protein self-organization
a Schematics of the patterned supported lipid bilayer (pSLB) placed in a stretch system compatible with confocal microscopy. The pSLB is obtained by plasma cleaning a PDMS membrane in presence of a TEM grid. Only the exposed PDMS becomes hydrophilic, and subsequent liposome deposition renders a SLB after buffer rinse. The non-exposed PDMS remains hydrophobic and a lipid monolayer is formed instead. b Representative images of the mechanical stimulation of the pSLB, showing both lipid and protein fluorescence images. In the resting initial state, excess liposomes stand on top of the pSLB. With strain, the liposomes incorporate in the pSLB. Upon release, excess lipids are expelled in the form or tubes or buds. At this stage, fluorescent Amphiphysin is gently microinjected on top of pSLB and its binding to the tubes and buds is monitored with time. c Membrane tubes (green inset) and buds (purple inset) before (left) and after (right) being reshaped by Amphiphysin. d Control in which no protein is injected on top of the pSLB. Scale bar, 5 μm.
