Fig. 3: Drivers of intratumoural heterogeneity in the malignant cell compartment of Vκ*MYC mice. | Nature Communications

Fig. 3: Drivers of intratumoural heterogeneity in the malignant cell compartment of Vκ*MYC mice.

From: Longitudinal single-cell analysis of a myeloma mouse model identifies subclonal molecular programs associated with progression

Fig. 3

a Heatmap of genome-wide copy number variations (CNVs) inferred from scRNA-seq data of malignant plasma cells as determined using InferCNV34. Columns represent genome position across chromosomes. Rows represent CNVs averaged by intra-sample CNV subpopulation, which were identified using the ward.D2 hierarchical clustering/random forest method implemented by analysis_mode = ‘subclusters’ in inferCNV. CNV-driven subpopulation sizes ranged from 1 to 979 cells (median 44). The height of each CNV subpopulation is proportionate to its fractional composition within a given tumour. b UMAP visualization of malignant cells from each active-MM mouse coloured by transcriptional cluster. Gene expression-driven cluster sizes ranged from 10 to 474 cells (median 79). c UMAP visualization of malignant cells from each active-MM mouse coloured by CNV subpopulation. d Bar plot showing the distribution of CNV subpopulations (fill) across transcriptional clusters (x-axis). Results for (b–d) are organized for each active-MM mouse in columns, with subject names and the number of cells/transcriptional clusters listed above. Source data are provided in SourceData_Fig. 3.xlsx. EMM: early-MM, IMM: intermediate-MM, AMM: active-MM.

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