Fig. 5: Mutations at key tertiary motifs affect the structure and Xrn1 resistance of flaviviral xrRNAs.

a–b The dimensionless kratky plots for G3C (a) and J2/3 (b) mutants of xrRNA1s from DENV2, ZIKV, and WNV in the presence of 5 mM EDTA (left) or 5 mM Mg²⁺ (right). c–d R_g (c) and D_max (d) for the wild type and J2/3 mutants of xrRNA1s from DENV2, ZIKV, and WNV in 5 mM Mg²⁺ or 5 mM EDTA. Each column in c represents an independent experiment with n = 1 and the error bars are propagated uncertainties calculated by GNOM. e–g The relative Xrn1 decay rates for the wild type, PK1, J2/3, and PK2-L3 mutants of DENV2-xrRNA1 (e), ZIKV-xrRNA1 (f), and WNV-xrRNA1 (g) at various Mg²⁺ concentrations. The decay rates were calculated by fitting the fluorescence traces (see supplementary Fig. 8d–l) with a single exponential curve. Data are presented as mean ± SEM. n = 3 biologically independent samples examined over three independent experiments in e–g. Source data for a, e–g are provided as a Source Data file.