Fig. 6: Myosin II suppresses cellular reorientation and lags behind Cdc42 activity response. | Nature Communications

Fig. 6: Myosin II suppresses cellular reorientation and lags behind Cdc42 activity response.

From: Directional reorientation of migrating neutrophils is limited by suppression of receptor input signaling at the cell rear through myosin II activity

Fig. 6

a Stacked bar plots of the percentage of cells exposed to transient stimulation that showed no response, medium response, strong response, and reversed for n = 261 cells of the Cdc42 line and n = 147 cells of the myosin line (from 20 and 8 independent experiments, respectively); p-value of two-sided Fisher’s exact test (**p < 0.01, exact p-value is provided in Source Data file). b Representative live-cell imaging snapshots of stimulation experiments with cells expressing parapinopsin and a myosin light chain sensor/cytosolic tag, showing a strong response (upper panel) and a reversed response (lower panel). Cells migrated unperturbed for 60 s prior to starting a transient 12-pulse stimulation at their cell rear (magenta circles). Myosin intensity pseudo-colored to facilitate visualization. Images  were captured every 3 s and subsampled for illustration purposes. Scale bar: 25 μm. c–e Mean cell centroid speed (c), normalized magnitude (d), and normalized derivative (e) of myosin intensity at the original front (blue) and rear (red) over time for each cellular response (lines: means, shaded regions: SD, error bars: mean value ± SE). Data are averages from n = 98 non-responders, n = 35 medium responders, n = 9 strong responders, and n = 5 reversers. Rectangular yellow shaded region represents the start and stop of the pulsated stimulation. Source data are provided as a Source Data file.

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