Fig. 1: Cell populations identified in developing kidneys with different genetic makeup are indistinguishable.

A tSNE of E14 Six2^KI data identifies 18 clusters. Annotation of clusters based on similarity to kidneys of the same genotype analyzed by Combes et al.⁴⁹ B GO-Elite analysis of Combes’ (black, C0-C14) and our (blue, C0-C17) Six2^KI clusters against global datasets. Note the cortical bias in our clusters. C UMAP clustering of all six samples. Gene expression patterns were merged in photoshop (i) to illustrate the temporal progression from naive (Cited1+, right) to epithelial (Gata3+, Hnf4a+, Mafb+) at left. Wnt4 marks transitional primed NPC. Cell-cycle states and cell type identities are shown in (ii), and the genotype contribution to each super-cluster in (iii). Note that contaminating erythrocytes are contributed predominantly by the green sample, E14 Six2^TGC;Tsc1 (color key in Fig. 2L).