Fig. 1: Unraveling CtBP2 cistrome leads to an identification of CtBP2/FoxO1 complex. | Nature Communications

Fig. 1: Unraveling CtBP2 cistrome leads to an identification of CtBP2/FoxO1 complex.

From: The transcriptional corepressor CtBP2 serves as a metabolite sensor orchestrating hepatic glucose and lipid homeostasis

Fig. 1

a–c CtBP2 ChIP-seq analysis in normal mouse liver tissues (6-h fasted). a Gene ontology analysis. Upper: biological process, lower: BioCyc pathway. b ChIP-seq peaks at representative metabolic gene loci. c Motif analysis of CtBP2 binding sites. The motifs enclosed by a rectangle are CtBP2-binding motifs enriched in the ChIP-seq. Prediction of known transcription factors targeted by CtBP2 based on sequence similarity is shown below. The statistical evaluation was performed based on the specific algorithm85. d Mutation of the PSDL motif in FoxO1(Mut: PSDL > PSAS) diminishes CtBP2/FoxO1 interaction in HEK293 cells. e, f Primary hepatocytes were stimulated with vehicle or 2 μM forskolin (fsk) after knockdown (e) or overexpression (f) of CtBP2. e The effect of CtBP2 and/or FoxO1 knockdown on G6pc expression (n = 5, biologically independent cells). The y-axis scale is expanded in the inset to show the data in the absence of fsk. The p values are as follows: 0.011 for sh-cont vs sh-CtBP2, 0.036 for sh-CtBP2 vs sh-FoxO1, 0.022 for sh-cont/fsk vs sh-FoxO1/fsk, 1.6 × 10−4 for sh-cont/fsk vs sh-CtBP2/fsk, 9.4 × 10−4 for sh-CtBP2/fsk vs sh-FoxO1/CtBP2/fsk. f G6pc expression following CtBP2 overexpression (n = 4, biologically independent cells). The p values are as follows: 5.6 × 10−4 for GUS vs CtBP2, 5.6 × 10−5 for GUS/fsk vs CtBP2/fsk. Data are expressed as the mean ± SEM. * and ** denote p < 0.05 and p < 0.01 evaluated by unpaired two-tailed Student’s t-test, respectively. Source Data are provided as a Source Data file.

Back to article page