Fig. 5: CtBP2 deficiency in liver leads to obesity-associated metabolic disturbances.
a Liver-specific CtBP2-deficient mice (KO) maintained on a regular chow diet (a–h) or an MCD diet for a week (h, i) (6–8 weeks of age, a, c, e–i: 6-h fasted, b, d: overnight fasted) were analyzed along with their wild-type controls (WT). a CtBP1 and CtBP2 expression at protein levels in either liver or adipose tissues. b Glucose tolerance test (GTT) (n = 16 for WT and n = 8 for KO, biologically independent animals, p values are as follows: 4.3 × 10−3 for 0 min, 4.4 × 10−4 for 15 min, 2.2 × 10−4 for 30 min, 2.0 × 10−6 for 60 min, 2.6 × 10−5 for 90 min, 8.1 × 10−3 for 120 min). c Gluconeogenic gene expression (n = 19 for WT and n = 9 for KO, biologically independent animals, p = 2.5 × 10−4 and 1.0 × 10−3 for G6pc and Pck1, respectively). d Pyruvate tolerance test (PTT) (n = 12 for WT and n = 6 for KO, biologically independent animals, p values are as follows: 1.2 × 10−3 for 0 min, 1.9 × 10−3 for 15 min, 3.1 × 10−3 for 30 min, 3.5 × 10−4 for 60 min, 1.9 × 10−5 for 90 min, 1.4 × 10−4 for 120 min). e Liver triglyceride (TG) content (n = 11 for WT and n = 9 for KO, biologically independent animals, p = 0.023). f Lipogenic gene expression (n = 8 for WT and n = 9 for KO, biologically independent animals). g Expression levels of FoxO1 and SREBP1 at protein levels. h Serum ALT levels in mice fed either a regular chow diet (RD) or an MCD diet for a week (n = 14 for WT/RD, n = 10 for KO/RD, n = 9 for WT/MCD, n = 8 for KO/MCD, biologically independent animals, p = 0.048 for WT vs KO on an MCD diet). i Liver triglyceride (TG) content in mice fed an MCD diet for a week (n = 13 for WT and n = 8 for KO, biologically independent animals, p = 8.3 × 10−5). Data are expressed as the mean ± SEM. * and ** denote p < 0.05 and p < 0.01 evaluated by unpaired two-tailed Student’s t-test, respectively. Source Data are provided as a Source Data file.
