Fig. 2: PARP1 activity facilitates DNA binding of ZNF384 at sites of damage.

a GFP-ZNF384 recruitment to 800 nm multiphoton tracks in stable U2OS Flp-In/T-Rex cells treated with PARP inhibitor (PARPi) for 1 h before micro-irradiation (top panel). Quantification of the data is presented as the mean ± SEM of >35 cells acquired in three independent experiments (bottom panel). b ZNF384 recruitment to 365 nm UV-A tracks 10 min after DNA damage induction in BrdU-sensitized wild-type (WT) and the indicated KO U2OS cells (left panel). The mean ± SEM of >180 cells from three independent experiments is shown (right panel). c Confocal images showing accumulation of GFP-WWE at sites of 405 nm laser micro-irradiation in Hoechst-sensitized U2OS cells. Cells were left untreated or treated with PARPi 180 s after DNA damage induction. d Boxplot limits correspond to the 25th and 75th percentiles and the center line in the box indicates the median value of the accumulation of GFP-WWE at 450 s post irradiation from 23–25 cells from a representative of three independent experiments. e As in (c), except for GFP-ZNF384. f As in (d), except for GFP-ZNF384 from 21–27 cells. Boxplot limits correspond to the 25th and 75th percentiles and the center line in the box indicates the median value. The whiskers extend 1.5 times the interquartile range. g GFP-ZNF384 recruitment to 405 nm laser tracks in U2OS cells overexpressing iRFP-ALC1 wild-type (WT) and iRFP-ALC1 ATPase-dead (E175Q) treated with PARPi for 1 h before micro-irradiation (left panel). GFP-ZNF384 recruitment is displayed as intensity integrated over the damaged area (right panel). The mean ± SEM from 13–16 cells from a representative of three independent experiments is shown. White triangles indicate irradiated regions. Scale bar 5 μm. All P values were calculated using the unpaired Student’s t test, assuming unequal variances. Source data are provided as a Source Data file.