Fig. 6: Rescue of dpp mutant by cell-autonomous Dpp signaling.
From: Asymmetric requirement of Dpp/BMP morphogen dispersal in the Drosophila wing disc
a, b α-pMad, α-Brk, α-Sal, α-Omb, 5xQE.DsRed, and DSRF staining of a dppd8/dppd12 wing disc and b dppd8/dppd12, dpp > tkvQD wing disc. Arrows indicate rescued posterior wing pouch identified by 5xQE.DsRed and DSRF staining. c Adult wing of dppd8/dppd12. d Adult wing of dppd8/dppd12, dpp > tkvQD. e A schematic view of converting dpp-Gal4 into a LexA driver, which is permanently expressed in lineage of dpp-Gal4 expressing cells. In this experimental setup, the lineage of dpp-Gal4 (including lineage of non-specific dpp-Gal4 expression) will permanently activate TkvQD and thus pMad signaling. f α-pMad, α-Brk, α-Sal, α-Omb staining, and 5xQE.DsRed signal of dppd8/dppd12, dpp > FLP, act > y + >LexA-LHG, LOP-tkvQD wing disc. Arrow indicates rescued posterior wing pouch identified by 5xQE.DsRed staining. Scale bar 50 μm.
