Fig. 7: Initial uniform dpp transcription in the anterior compartment.
From: Asymmetric requirement of Dpp/BMP morphogen dispersal in the Drosophila wing disc
a A schematic view of a Gal4 insertion into the dpp locus. b A schematic view of G-TRACE analysis. While RFP expression labels the real-time Gal4-expressing cells, GFP expression labels the lineage of the Gal4-expressing cells. c G-TRACE analysis of the endogenous dpp-Gal4. Scale bar 50 μm. d A schematic view of d2GFP insertion into the dpp locus. e−h α-GFP and α-Ptc/Wg staining of wing disc expressing the d2GFP reporter at mid-second instar stage (60 h AEL) (e), at early third instar stage (72 h AEL) (f), at mid-third instar stage (84 h AEL) (g), and at mid- to late- third instar stage (96 h AEL) (h). eʹ−hʹ Magnified wing discs from (e−h). Arrows indicate dpp transcription outside the stripe of cells. Scale bars as indicated. i−l smFISH against dpp using RNA scope technology. yw wing disc at 60 h AEL (i), 72 h AEL (j), 84 h AEL (k), 96 h AEL (l). iʹ−lʹ Magnified wing discs from (i−l). Scale bars as indicated.
