Fig. 5: Differential transcription factor activity orchestrates LPS responses in Scnn1b-Tg AMs. | Nature Communications

Fig. 5: Differential transcription factor activity orchestrates LPS responses in Scnn1b-Tg AMs.

From: Epigenetic reprogramming of airway macrophages promotes polarization and inflammation in muco-obstructive lung disease

Fig. 5

a Differential transcription factor (TF) activity analysis of lipopolysaccharide (LPS) treated Scnn1b-transgenic (Tg) vs. wild-type (WT) airway macrophages (AM). A positive weighted mean difference indicates increased TF activity in Scnn1b-Tg AMs. Size indicates the number of TF binding sites (TFBS). Green labeled TFs are predicted activators, red labeled TFs are predicted repressors, and black labeled TFs have no direction assigned. b Pearson correlation of TF activity of significantly enriched TFs (adjusted (adj.) P value < 0.001) in Scnn1b-Tg vs. WT AM treated with LPS on the y-axis, with mean target gene expression change on the x-axis. The gray diagonal represents the linear regression. c Clustering of differentially activated TFs (adj. P value <10e−6) based on the similarity of their position weight matrixes. Coloring of tree leaves based on TF activity. Coloring of labels defined by TF class. d Normalized Irf1 gene counts obtained by RNA sequencing (RNAseq) (Scnn1b-Tg LPS vs. WT LPS) in treated and untreated Scnn1b-Tg and WT AMs. Adj. P values were determined by DESeq2. The data show mean ± SEM of n = 3. Source data are provided as a Source Data file.

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