Fig. 3: SETD2 binds to hnRNP LL through its SHI Domain. | Nature Communications

Fig. 3: SETD2 binds to hnRNP LL through its SHI Domain.

From: Structural basis of the interaction between SETD2 methyltransferase and hnRNP L paralogs for governing co-transcriptional splicing

Fig. 3: SETD2 binds to hnRNP LL through its SHI Domain.The alternative text for this image may have been generated using AI.

a, d, h Cartoon illustrating the domain organization of hnRNP L, hnRNP LL, SETD2, and ySet2. b, c Heat maps showing the enrichment of proteins normalized to the bait (SETD2C) in MudPIT analysis. e, f, j Western blotting and silver staining of affinity-purified complexes of Halo-SETD2C or Halo-ySet2 and its mutants from 293 T extracts. The experiment was repeated at least three times all yielding similar results. Source data are provided as a Source Data file. g, i Table showing the dNSAFs of the listed proteins post mass spectrometry analysis of purified complexes obtained by affinity purification of Halo-SETD2 or ySet2 from 239 T extracts. AWS associated with SET, SET-Su(var)3-9 enhancer-of-zeste and trithorax, SRI–Set2–Rpb1 interaction, SHI– SETD2–hnRNP interaction dNSAF distributed normalized spectral abundance factor, NLS nuclear localization signal.

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