Fig. 7: Heatmap of transcriptional genes of the hypoxia pathway following SARS-CoV-2 infections.

K18-hACE2 mice of both sexes (~1:1 ratio) were infected intranasally with USA-WA1/2020 (WA) of lineage A bearing 614D (104 TCID50/mouse), New York-PV09158/2020 (NY) of lineage B.1.3 bearing 614G (103 TCID50/mouse), USA/CA_CDC_5574/2020 (CA) of lineage B.1.1.7 (102 TCID50/mouse) or hCoV-19/South Africa/KRISP-EC-K005321/2020 (SA) of lineage B.1.351 (102 TCID50/mouse). Hypoxia signaling pathway PCR array was performed using RNA extracted from lung homogenates of naïve or infected mice at day 3 post infection. Gene regulation as fold changes over naïve mice was used to construct the heatmap and the fold changes of individual genes are summarized in Supplementary Table 1. WA (614D), n = 5 mice/group; NY (614G), n = 4 mice/group; CA (B.1.1.7), n = 4 mice/group; SA (B.1.351), n = 4 mice/group; naïve, n = 4 mice/group.