Fig. 5: SUMO-dependent interactors of PML localize to PML NBs.
From: Identification of proximal SUMO-dependent interactors using SUMO-ID
Confocal microscopy analysis of PML SUMO-ID identified proteins in U2OS YFP-PML knock-in cell line. UBC9 was added as an expected positive control. Yellow arrowheads indicate colocalization events. Dotted line-squares show the selected colocalization events for digital zooming and the signal profile plotting shown to the right. Nuclei are stained with DAPI (blue), YFP-PML is shown in green and the indicated proteins in magenta. Black and white panels show the single green and magenta channels. Images are representative of three independent stainings performed into the same YFP-PML cell line. Further colocalization analysis using the Colocalization Colormap and JACoP is provided as Supplementary Fig. 8. Arb. units Arbitrary units. Scale-bar: 5 μm.
