Fig. 6: Characterization of the whole PML NBs proteome.
From: Identification of proximal SUMO-dependent interactors using SUMO-ID
a Volcano plot of LC-MS analysis comparing streptavidin pull-downs of U2OS stable cell lines for TurboID-PMLWT or TurboID alone. Cells were treated with 50 μM of biotin for 2 h. High-confidence PML proteome composed of 271 proteins is shown as blue dots. Statistical analyses were performed by two-sided Student’s t test. Data are provided as Supplementary Data 3. b STRING network analysis of the whole PML NBs proteome defined in a shows a high interconnected network composed of the 73.6% of the proteins. Highly interconnected sub-clusters were characterized using MCODE. Color, transparency and size of the nodes were discretely mapped to the Log2 enrichment value as described. The border line type was discretely mapped to the p value as described. c Gene ontology analysis of the whole PML NBs proteome defined in a. Depicted biological processes, molecular functions and cellular components were significantly enriched. Dotted line represents the threshold of the p value (0.05). Data are provided as Supplementary Data 4.
