Fig. 5: Robust and prolonged glycocalyx engineering using Chol-terminal GalNAcS- glycobrushes.
From: Tunable, biodegradable grafting-from glycopolypeptide bottlebrush polymers
a Reaction scheme for conjugation of AF594-NHS to glycobrush (PLG0.8-s-PAIK0.2)50-g-PGalNAcS13-Chol amino termini, followed by engineering of the glycocalyx of live epithelial cells. Cell images in b, c are 24 h after incubation b with 15 µM AF594-glycobrush-N3 or c with 15 µM AF594-glycobrush-Chol. d Flow cytometry data of HEK293T cells untreated (blue) or treated with AF594-glycobush-N3 (magenta) or AF594-glycobrush-Chol (black). Cell images in e–g are paraformaldehyde-fixed HEK293T cells that were incubated with 15 µM AF594-glycobrush-Chol and 30 μg/mL CF488A-transferrin, where e is imaging for AF594-glycobrush-Chol, f is imaging for CF488A-transferrin, and g is the overlay of the images in e and f. Scale bars are 50 µm. Images in b, c, e–g are representative of four separate experiments.
