Fig. 4: Genome-wide associations of PBAF in spermatocytes.
From: Mammalian SWI/SNF chromatin remodeler is essential for reductional meiosis in males
a Schematic illustrating protocol used to synchronize spermatogenesis using WIN 18,446 and retinoic acid (RA), b Heatmap displaying the average enrichment of ARID2 and BRG1 relative to no IgG control from spermatocyte populations isolated at P28 and P28.5. Enrichment is displayed over a 4 Kb window centered at transcription start sites (TSS’s) of RefSeq genes. K-means clusters (Cl-1 and 2) are based on ARID2 enrichment. c ChIP-qPCR analysis of ARID2, BRG1, H3T3P, and H3K27me3 enrichment at centromeric minisatellites (Mini-Sat) and pericentric major satellites (Major-Sat) repeats. Fold enrichment (y-axis) were calculated relative to known gene desert (MMU19) and were expressed as mean ± SEM. Dots (black) represent values obtained from three independent experiments.
