Fig. 4: Structural changes of Pat-1 during the lag phase.
From: Dissection of the amyloid formation pathway in AL amyloidosis
a Change of tryptophan fluorescence. Each emission spectrum is the average of three individual scans. b Change of surface hydrophobicity followed by ANS fluorescence. c Change of tryptophan fluorescence upon quenching with 0–1 M acrylamide. The curves show the respective F0/F values of the Stern-Volmer equation, Error bars represent the SEM of n = 3. d Comparison of the normalized change of ANS fluorescence (gray), Trp exposure (orange) and the quenching constant K of the acrylamide quenching calculated by Eq. (1) (purple) in comparison to changes in the ThT signal (pink). Shades represent the SEM of n = 3.
