Fig. 4: Evolution and expansion of terpene synthase genes and contraction of R genes in Chloranthales.
From: Chloranthus genome provides insights into the early diversification of angiosperms
a A total of 44 genes related to the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway and the mevalonate (MVA) pathway were identified in C. spicatus (left panel). HMGR and DXS exhibited the highest copy numbers in the MEP and MVA pathways, respectively. Differentially expressed genes among seven representative tissues of C. spicatus involved in MEP and MVA pathways are shown in the right panel. b Identification of candidate terpene synthases (TPSs) in C. spicatus and subfamily classification revealed six major clades (TPS-a, b, c, e, f, and g). The gene family tree indicates that TPS-a and TPS-b gene clades are significantly expanded in magnoliids and Chloranthales. c Contraction of R genes in Chloranthales. The nucleotide-binding site-leucine-rich repeat (NBS-LRR) genes were divided into three classes: TIR-NBS-LRR (TNL), CC-NBS-LRR (CNL), and RPW8-NBS-LRR (RNL). In all, 3,518 NBS genes were identified in 28 angiosperm species. ‘*’ indicates the data were obtained from a previous study40.
