Fig. 3: Functional characterization of AgmA as AMP phosphoribohydrolase.
From: Efficient biosynthesis of nucleoside cytokinin angustmycin A containing an unusual sugar system
a HPLC traces (λ = 254 nm) of the AgmA reactions using AMP or dAMP as the substrate. Std, the authentic standards of AMP, dAMP, and adenine; AgmA(AMP), AgmA reaction with AMP as substrate; -AgmA(AMP), reaction using AMP as substrate but without AgmA added; AgmA(dAMP), AgmA reaction with dAMP as substrate; -AgmA(dAMP), reaction using dAMP as substrate but without AgmA added; -AMP/dAMP, AgmA reaction without AMP or dAMP added. b Sequence similarity network (SSN) of 9058 AgmA homologs generated by Cytoscape (v3.8.2). Each node in the network represents a protein sequence and the alignment score is 70. AgmA falls into the actinobacteria cluster and is highlighted by a yellow point.
