Fig. 3: Affinity and specificity profiling of RB aptamer pools.
From: Discovery of indole-modified aptamers for highly specific recognition of protein glycoforms
A Round-to-round enrichment of the aptamer pool during particle display. Aptamer particles were incubated with fluorescently-labeled RB and analyzed via flow cytometry. The pre-enriched library (after 5 rounds of SELEX) was assayed at 6.8 µM RB, while the pools obtained after MPPD rounds 1 and 2 were assayed at 3.4 µM RB. The cytometry gates are set arbitrarily to the edge of the population and provide a consistent reference point between the plots. B Two-color binding assays to simultaneously assess enrichment of aptamer pools for both RA and RB. Aptamer particles were incubated with 3.4 µM fluorescently-labeled RB and RA and analyzed via flow cytometry. The lefthand plot shows the naïve library (before pre-enrichment), the righthand plot shows the final pool after two rounds of MPPD. Plots were generated using FlowJo.
