Fig. 1: Comprehensive dissection and clustering of single cells from MPE and blood.
From: Single-cell analysis of diverse immune phenotypes in malignant pleural effusion
a The flowchart of the overall study design. scRNA-seq and expression analysis of malignant pleural effusion (MPE) and blood samples (n = 5) were performed on the 10× Genomics platform. b t-SNE plots within each sample type, color-coded by cell types. c Average proportion of each cell type derived from each patient, color-coded by cell types. d Canonical marker genes for the immune cell types defined in Fig. 1b. Data are colored according to expression levels. e Dot plot of average expression of canonical marker genes for the immune cell types defined in Fig. 1b. f Gene ontology (GO) enrichment analysis using the genes upregulated in MPE compared with blood for each cell type. The statistical significance was tested by Fisher’s exact test and adjusted by Benjamini–Hochberg correction. g Frequencies of four immune cell types in MPE and blood according to the t-SNE plot using scRNA-seq data. Data are presented as mean ± SD. Comparisons were made using two tailed paired Student’s t test. Blood, n = 5 samples, MPE, n = 5 samples.
