Fig. 3: PYK2 phosphorylates IRF5.
From: Defactinib inhibits PYK2 phosphorylation of IRF5 and reduces intestinal inflammation
a Phosphorylation sites identified in LPS-stimulated WT and PYK2 KO RAW264.7 cells. b MS/MS spectrum of the IRF5 derived tryptic peptide 152-179 indicating phosphorylation at positions Y171. Fragmentation ions of the b- and y- series are indicated in blue and red, respectively. c In vitro kinase assay and immunoblot of IRF5-site-specific tyrosine mutants. HEKTLR4 cells were co-transfected with FLAG-IRF5 tyrosine mutants as indicated and Myc-PYK2. 10% lysate was kept for input and the remaining was used for in vitro kinase reactions. Kinase assays were detected by western blot using antibodies against Flag-(IRF5) and Myc-(PYK2). Representative blots from three independent experiments are shown. Source data are provided as a Source Data file.
