Fig. 1: Seroprofiling SARS-CoV-2 responses.

a Assessment of the multiplex SARS-CoV-2 ABCORA 2.0 on the indicated training (N = 823) and validation (N = 635) cohorts (Supplementary Table 3). Depicted are MFI signals normalized to empty bead controls (MFI-FOE). Gray boxes indicate values above the individually set MFI-FOE cut-offs for SARS-CoV-2 specific responses for each antigen (see Supplementary Table 4). Boxplots represent the following: median with the middle line, upper and lower quartiles with the box limits, 1.5x interquartile ranges with the whiskers. b Heatmap representing the measured MFI-FOE values and the outcomes predicted with ABCORA 2.0–2.3 of training and validation cohort measurements shown in (a). Negative, Positive, and Positive, partial refer to ranking according to ABCORA 2.0 as specified in Supplementary Table 5. c Sensitivity and specificity of ABCORA 2 assay versions based on the combined training and validation cohort data depicted in (a). Proportion of false negative samples (sensitivity; green) and proportion of false positive samples (specificity; blue) are represented by the reduction from 100% (outer circle) per segment. d Assessment of ABCORA 2.0 with the National Institute for Biological Standards and Control (NIBSC) Anti SARS-CoV-2 Verification Panel (20/B770) comprising SARS-CoV-2 positive (red) and negative (blue) panel serum samples. Gray boxes indicate values above the ABCORA 2.0 MFI-FOE cut-offs for SARS-CoV-2 specific responses for individual antigen-Ig combinations. Boxplots represent the following: median with the middle line, upper and lower quartiles with the box limits, 1.5x interquartile ranges with the whiskers.