Fig. 4: CryoEM structures of ADP-AlF4-bound M854K MDA5-dsRNA filaments.
a Superposition of two atomic models of ADP-AlF4-bound M854K MDA5-dsRNA filaments representing the most populated classes of 3D cryoEM image reconstructions. The class representing the majority of filament segments (Class 167k, cyan) contains 14 bp RNA in the asymmetric unit; the minority structural class (Class 62k, green) contains 15 bp RNA. b Atomic models and 3D density maps for the dsRNA for the two structural classes of ADP-AlF4-bound M854K MDA5-dsRNA filaments. Left, Class 167k, 6.8-σ contour level in PyMol; Right, Class 62k, 6.0-σ contour level. c Histograms showing the distributions of filament segments as a function of helical twist for M854K and WT MDA5-dsRNA with ADP-AlF4 bound. The twist distribution for WT is shown for reference and was reported previously14. The distributions are from 3D classifications performed with ten classes per dataset. Error bars represent the SEM between 3D classification calculations, centered on the mean (n = 3 independent calculations). Source data are provided as a Source Data file.
