Fig. 1: Room-temperature ODNP-enhanced 13C NMR of 13C2 imidazole in D2O at 9.4 T. | Nature Communications

Fig. 1: Room-temperature ODNP-enhanced 13C NMR of 13C2 imidazole in D2O at 9.4 T.

From: Room-temperature dynamic nuclear polarization enhanced NMR spectroscopy of small biological molecules in water

Fig. 1

a The chemical structure of imidazole molecule. The black circle indicates 13C labeled position. b Scheme of Fabry-Pérot resonator16,39. The sample (102 nL) has been laid as a thin layer on the flat mirror (gray). The sample diameter (Φ) and thickness (l) are shown. The microwave beam (shown as mw arrow) enters the resonator via a hole in the middle of a spherical mirror (gray). c Spectra obtained with (up) and without (down) microwave (mw) are presented. A 50-fold ODNP enhancement on 13C signal has been obtained. TEMPOL (100 mM) was used as the hyperpolarizing agent. The spectra are scaled with number of scans for visualizing directly the enhancement factor. The microwave-off spectra are further scaled up by 10-fold for the better visualization.

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