Fig. 3: Discrete trait mapping on time-scaled phylogeny of Cluster A.

Eight traits including host, farm ID (farm), province, the 4 unique sites in the spike protein with amino acid changes (L452M, Y453F, F486L and N501T) and the combinations of the 4 sites (combineAA) are mapped on the same MCC tree using the discrete trait model. The traits are plotted individually and for each tree, the branches and nodes are coloured by inferred ancestral traits. Samples which fell within mink clusters but not isolated from farms are highlighted in diamond (1 escaped mink and 1 un-associated human sequences). The outgroup containing human samples are cross labelled.