Fig. 2: Features of metastases in A3250 xenograft model.

a Metastatic burden in organs as indicated, determined by bioluminescent imaging (total Flux) ex vivo, 6 weeks post-injection (n = 9 mice). Inguinal draining lymph nodes (dLNs; n = 17), axillary and brachial non-draining lymph nodes (ndLNs; n = 35). Solid black lines indicate mean values. Red solid line indicates the threshold of positive Fluc signal above background. Representative images of lymph node (b) and lung (c) metastases (GFP, green) in tissue sections (DAPI, blue). Metastases in the lymph node stained by H&E (d) or Ki-67 proliferation marker (e, f). Lung metastases stained by H&E (g) or Ki-67 (h–j). Different sizes of metastases are depicted (h–j). k Weight loss of mice bearing A3250 tumors at six-week endpoint (n = 10 biologically independent mice). Differences between the true means were determined through a one-sample t test (p < 0.0001). Short black line indicates mean % weight loss. Red dotted line indicates the average weight gain of an age-matched, naïve control mouse during the same period. p value was determined by one sample t test. Images are representative of two independent experiments. n = 5 biologically independent mice. ***p < 0.001. Scale bars: b, e, 200 μm; c, d, f–j, 100 μm. Source data are provided as a Source Data file.