Fig. 6: Lung inflammation in mice bearing A3250 tumors.

a–h Lungs of mice bearing A3250 or MDA-MB-231 tumors at 4 weeks post-injection, and tumor-naïve lungs from age-matched SCID mice as indicated. Sections were stained with H&E (a–d) or immuno-stained for CD45 (e–h). i Frequency of myeloid cell subsets in lungs from A3250 tumor-bearing and naïve mice determined by flow cytometry (%Ly6C: p = 0.09, %Ly6G: p = 0.0146). n = 5 lungs in each experimental group. j Frequency of myeloid cell subsets in blood from tumor-bearing and naïve mice as indicated, determined by flow cytometry (%CD11b⁺: p = 0.00373; %Ly6C⁺: p = 0.00629; %Ly6G⁺: p = 0.00728). n = 5 blood samples for naïve mice and 6 for mice with A3250 tumors A two-tailed t test was performed to determine significance (i, j). k Ratio of myeloid cell subsets in lungs as indicated. Black line on the plots indicates mean ± SEM. Scale bars: (a), (e), 1 mm; (b–d), (f–h), 100 μm.**p < 0.01 *p < 0.05; ns, not significant. Source data are provided as a Source Data file.