Fig. 4: Crucial role of Tyr35 of ISCU2 in late but not early steps of core ISC complex function.
From: N-terminal tyrosine of ISCU2 triggers [2Fe-2S] cluster synthesis by ISCU2 dimerization
a Affinities of the indicated ISCU2 proteins for wild-type (NIA)2 were measured by microscale thermophoresis (the data for wild-type ISCU2 was taken from Fig. 3a). The numbers within the bars represent Kd values (error bars represent SD of the fit using three independent experiments, Supplementary Fig. 8a). b Determination of DTT-mediated sulfide (S2-) production by the (NIA)2 complex in the presence of the indicated ISCU2 proteins without and with FXN after 20 min of incubation (n = 3 biological replicates, individual data points are shown, error bars show SD). c Persulfide transfer from NFS1 onto the indicated ISCU2 proteins was assayed as in Fig. 3c. d Enzymatic [2Fe-2S] cluster reconstitution (cf. Fig. 3d) of the indicated ISCU2 proteins with wild-type (NIA)2. Source Data are provided as a Source Data file.
