Fig. 1: Lipopeptide antibiotic structures and organization of NRPS assembly lines.
From: Gene editing enables rapid engineering of complex antibiotic assembly lines
a Structures of wild type and engineered enduracidins; amino acids in blue were targeted for modification, with organization of the enduracidin NRPS shown below. b Structure of ramoplanin A2 and organization of the ramoplanin NRPS. Nonproteinogenic amino acid abbreviations: 4-hydroxyphenylglycine (Hpg), ornithine (Orn), allo-threonine (aThr), citrulline (Cit), enduracididine (End) and 3,5-dichloro-4-hydroxyphenylglycine (Cl2-Dpg), 3-chloro-4-hydroxyphenylglycine (Cl-Hpg). †Titres of engineered products 2–6 relative to 1 produced by the wild type S. fungicidicus.
