Fig. 4: Overview of the primary (Swap 1–10) subdomain swap mutants generated in this study.
From: Gene editing enables rapid engineering of complex antibiotic assembly lines
Most of the subdomain swaps were designed for endC, the largest NRPS, containing multiple repeating sequences, which presents a significant challenge for engineering conventional homologous recombination methodologies. *Swap via gene complementation, † Relative titre of engineered variants (2–6) compared titres of 1 produced by wild type, ‡ selectivity for new products (2–6) over parent enduracidin (1) in engineered NRPS. Coloured arrows are used to indicate different amino acid swaps: green (Thr[2] to Ser), blue (Ser[12] to Thr), pink (Ser[12] to aThr), brown (Ser[12] to Ala), purple (Gly[14] to Ala) and yellow (Ala[16] to Gly). Source data for yield and selectivity are provided with this paper.
