Fig. 6: Titres of enduracidin analogues produced by FSD swap mutant.
From: Gene editing enables rapid engineering of complex antibiotic assembly lines
Production levels (mg/L) for engineered enduracidin from FSD swaps compared with the wild type enduracidin (1a & 1b). Non-filled bars represent a batch of ten replicates (n = 10) for Swaps 1–5 and Swaps 8–10. Shaded bars represent a different batch of cultures carried out in triplicate (n = 3) for Swaps 12, 15, 16 and 17. Data are presented as mean values + /− standard error. Each data point is overlaid on the graph as a dot plot. The wild type strain is included in both batches as a positive control. The production for Swap 10 was calculated from an average of eight samples, omitting two statistical outliers from flasks 1 and 5. The ratio of enduracidin a & b variants produced by wild type and engineered strains is variable and so the combined yields (a & b) are reported in each case to enable a clearer comparison of the productivity of each strain. Each bar is colour-coded for the analogues produced: green (2a & 2b), blue (3a & 3b), pink (4a & 4b), purple (5a & 5b) and yellow (6a & 6b). Source data are provided with this paper.
