Fig. 4: Dnmt1 deficiency in brown fat induces BAT-to-myocyte remodeling.
From: Epigenetic interaction between UTX and DNMT1 regulates diet-induced myogenic remodeling in brown fat
A Heatmap of myogenic gene expression in iBAT from D1KO and fl/fl mice on regular chow diet. B Quantitative RT PCR analysis of myogenic gene expression in D1KO and fl/fl mice on chow diet (n = 5/group). *Indicates statistical significance between fl/fl and D1KO by Mann–Whitney’s nonparametric U test. C Hierarchical cluster analysis of genes similarly upregulated in iBAT of HFD-fed, UTXKO and D1KO mice. D Representative IHC staining of MyHC in GFP-labeled brown adipocytes in iBAT of D1KO-GFP and fl/fl-GFP mice on chow diet (n = 3 replicates per group). E Quantitative RT-PCR analysis of BAT-specific gene expression in iBAT of D1KO and fl/fl mice on chow diet (n = 5/group). *Indicates statistical significance between fl/fl and D1KO by unpaired two-tailed Student’s t-test. F Immunoblotting analysis of UCP1 protein levels in iBAT of D1KO and fl/fl mice on chow diet (n = 5/group). *Indicates statistical significance between fl/fl and D1KO by unpaired two-tailed Student’s t-test. G Representative IHC staining of UCP1 in iBAT of D1KO and fl/fl mice on chow diet (n = 3 replicates per group). H OCR of primary brown adipocytes isolated from iBAT of D1KO or fl/fl mice measured by a Seahorse analyzer (n = 14 for fl/fl, 20 for D1KO). *Indicates statistical significance between fl/fl and D1KO by unpaired two-tailed Student’s t-test. I Bioinformatic modeling of BAT-like or WAT-like gene expression profiles using RNA-seq data from iBAT of D1KO and fl/fl mice on chow diet using an online software (https://github.com/PerocchiLab/ProFAT). J BAT-specific gene expression in iBAT of D1KO and fl/fl mice on chow diet using an online software (https://github.com/PerocchiLab/ProFAT). The WAT reference aggregate and BAT reference aggregate were derived from the online software. All data are expressed as mean ± SEM.
