Fig. 6: DNMT1 deficiency in brown fat impairs cold-induced thermogenesis.
From: Epigenetic interaction between UTX and DNMT1 regulates diet-induced myogenic remodeling in brown fat
A Body temperature in male chow-fed 2-month-old D1KO and fl/fl mice during acute 5 °C cold exposure (n = 6 for fl/fl and 5 for D1KO). *Indicates statistical significance between fl/fl and D1KO as analyzed by two-tailed unpaired Student’s t-test. B Body temperature change in male chow-fed 2-month-old D1KO and fl/fl mice after 3 h of cold exposure (n = 6 for fl/fl and 5 for D1KO). *Indicates statistical significance between fl/fl and D1KO as analyzed by two-tailed unpaired Student’s t-test. C, D Quantitative RT-PCR analysis of BAT-specific gene expression (C, n = 6 for fl/fl and 8 for D1KO), and myogenic gene expression (D, n = 7 for fl/fl and 6 for D1KO) in iBAT of male chow-fed 2-month-old D1KO and fl/fl mice after an acute 4-h 5 °C cold exposure. *Indicates statistical significance between fl/fl and D1KO as analyzed by two-tailed unpaired Student’s t-test in (C) and Mann–Whitney’s nonparametric U test in (D). E, F Quantitative RT-PCR analysis of BAT-specific gene expression (E, n = 6/group), and myogenic gene expression (F, n = 6/group) in iBAT of male chow-fed 2-month-old D1KO and fl/fl mice after a chronic 7-day 5 °C cold exposure. *Indicates statistical significance between fl/fl and D1KO as analyzed by two-tailed unpaired Student’s t-test in (E) and Mann–Whitney’s nonparametric U test in (F). G Immunoblotting of UCP1 protein in iBAT of male chow-fed 2-month-old D1KO and fl/fl mice after a 7-day 5 °C cold exposure (n = 4/group). *Indicates statistical significance between fl/fl and D1KO as analyzed by two-tailed unpaired Student’s t-test. H, I Representative H&E staine (H) and UCP1 IHC staining (I) in iBAT from male chow-fed 2-month-old D1KO and fl/fl mice after a chronic 5 °C 7-day cold exposure (n = 3 replicates for each group). All data are expressed as mean ± SEM.
