Fig. 1: The genetic organisation, biochemistry and EM analysis of the F plasmid outer membrane complex.
From: Architecture of the outer-membrane core complex from a conjugative type IV secretion system
a The native arrangement of T4SS-associated genes within the pED208 tra operon with the arrows representing the genes encoding for the T4SS apparatus (green), relaxosome components (blue), mating pair stabilisation and entry exclusion (red), pilus biogenesis and dynamics (purple), and regulation (brown). b The primary sequence structure of the three proteins TraV (purple), TraK (green) and TraB (cyan) including their domains and boundaries. c SDS-PAGE of the OMCC with the bands of the proteins TraB (VirB10), TraK (VirB9) and TraV (VirB7). d Negative stained EM micrograph of the OMCC. Scale bar 100 nm. e Cryo-EM micrograph of the OMCC, with particles highlighted with green circles. Scale bar 100 nm. f Representative top-, tilted- and side-view 2D class averages obtained using CryoSparc v3.1.0 (see 'Methods' section for detailed information).
