Table 1 Cryo-EM data collection, processing and model refinement statistics.
From: Architecture of the outer-membrane core complex from a conjugative type IV secretion system
OMCC inner ring | OMCC outer ring | OMCC C1 | |
|---|---|---|---|
Data collection and processing | |||
Microscope | Titan Krios | Titan Krios | Titan Krios |
Camera | K3 | K3 | K3 |
Voltage (kV) | 300 | 300 | 300 |
Electron exposure (e- Å−2) | 50 | 50 | 50 |
Pixel size (Å) | 1.08 | 1.08 | 1.08 |
Defocus range (μm) | −0.7 to −3.0 | −0.7 to −3.0 | −0.7 to −3.0 |
Symmetry imposed | C17 | C13 | C1 |
Initial particle images | 1,445,958 | 1,445,958 | 1,445,958 |
Final particle images | 74,956 | 298,235 | 95,177 |
FSC threshold | 0.143 | 0.143 | 0.143 |
Map resolution (Å) | 3.3 | 3.4 | 5.7 |
Map B factor | −74 | −93 | −304 |
EMDB code | EMD-12962 | EMD-12963 | EMD-13231 |
Refinement and model validation | |||
Model versus data CC (mask) | 0.72 | 0.75 | n/a |
Clash score | 13.73 | 13.82 | |
MolProbity score | 2.18 | 2.25 | |
Bond lengths rmsd (Å) | 0.007 | 0.005 | |
Bond angle rmsd (°) | 1.136 | 1.033 | |
Poor rotamers (%) | 0 | 0 | |
Ramachandran | |||
Favoured (%) | 90.7 | 88.03 | |
Allowed (%) | 9.3 | 11.97 | |
Outlier (%) | 0 | 0 | |
PDB code | 7OKN | 7OKO | |
