Fig. 6: LSD1 loss unleashes TCF1-controlled gene expression in CD8⁺ TILs.

a A volcano plot showing differential gene expression by LSD1-deficient versus wildtype CD8⁺CD44⁺PD-1⁺ TILs of MC38 tumors (n = 4 per group, FC > 1.5 and FDR < 0.01 as the cutoff). Non-gray colored dots mean significant difference. b Percentages of genes bound by TCF1 among the differentially expressed genes in LSD1-deficient versus wildtype CD8⁺ TILs or among total genes in genome. c GSEA analysis in Lsd1 KO versus WT CD8⁺ TILs (n = 4 per group). Gene list was ranked with signed (from log₂FC) likelihood ratio from Lsd1 KO versus WT comparison. Kolmogorov–Smirnov test was used to determine the enrichment score. d, e A representative flow plot of IL7Rα expression (d) and IL7Rα MFI (e) of MC38 tumor-infiltrating CD8⁺PD-1⁺ T cells (n = 4 per group). f, g A representative flow plot of Slamf6 expression (f) and Slamf6 MFI (g) of MC38 tumor-infiltrating CD8⁺PD-1⁺ T cells (n = 4 per group). Data represent two independent experiments (d–g) and are presented as mean ± SEM (e, g). Statistical significance was determined by two-sided unpaired t test (e, g). Source data are provided as a Source data file.