Fig. 2: HSCs with short telomeres are persistently activated, overexpress genes involved in IFN signaling, and are poised towards megakaryocytic differentiation.

a UMAP of scRNA-seq data displaying 752 and 816 pooled single HSCs isolated from G0 or G5/G6 mice, respectively (n ≥ 2 mice per group). Each dot represents one cell. Different colors represent the sample (left) and cluster (middle) identities. Right, the distribution of HSCs from G0 and G5/G6 mice among the five scRNA-seq clusters, represented as the percentages of cells belonging to each cluster. b Left, pathway enrichment analysis of the marker genes of cluster 1 shown in Fig. 2a and Supplementary Dataset 3 (adjusted P ≤ 0.05). The Reactome gene sets are shown. Right, the dot plot shows the differential expression of the genes involved in IFN signaling across the five HSC clusters. c UMAP of scATAC-seq data displaying 1416 and 2046 pooled single HSCs isolated from G0 or G5/G6 mice, respectively (n ≥ 9 mice per group). Each dot represents one cell. Different colors indicate the sample origins (left) and cluster identities (right). d Dot plot of the level of the activities of the TFs whose binding sites were differentially enriched in the open chromatin regions of G5/G6 cells from cluster 0 as compared to those enriched in the open chromatin regions of G0 cells. Source data are provided as a Source Data file.