Fig. 3: Conformational changes in hDisp1 induced by proteolytic cleavage. | Nature Communications

Fig. 3: Conformational changes in hDisp1 induced by proteolytic cleavage.

From: Structural insights into proteolytic activation of the human Dispatched1 transporter for Hedgehog morphogen release

Fig. 3: Conformational changes in hDisp1 induced by proteolytic cleavage.

a Overall comparison of cryo-EM density maps for hDisp1NNN-3C, before (gray) and after cleavage (blue) by 3C protease. Proteolytic cleavage causes the two halves of the extracellular domain, ECD1 and ECD2, to move apart. b Two close-up views of the model for hDisp1, before (gray) and after cleavage (ECD1 yellow, ECD2 green). c As in (a), but showing hDisp1 models. d Close-up showing changes in the C-loop after 3C protease cleavage. Density maps before and after cleavage are represented as cyan and purple mesh, respectively. e Cryo-EM density map of uncleaved hDisp1 (left) shows extra density (red dotted oval), compared to cleaved hDisp1 (right).

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