Fig. 1: Structure of the FSDR and molecular analysis of VviPLATZ1.
From: VviPLATZ1 is a major factor that controls female flower morphology determination in grapevine
a Diagram of the SDR structure derived from the Cabernet Sauvignon f-haplotype29. Genes localized within the FSDR are highlighted in yellow32. Gray arrows depict genes located outside of the FSDR. Black arrows were used to display genes flanking the SDR. b Fine genetic mapping narrowed the FSDR to seven genes, highlighted in yellow. The black vertical arrows indicate the location of the VvMT54 and VvMT72 SNPs in TPP and FMO3, respectively, which mark the boundaries of the FSDR. c Transcript levels determined for VviPLATZ1 in 04C023V0006 (H/H) and 04C023V0003 (f/f) flowers at eight, six and four weeks prior to anthesis (WPA), as well as leaves (L). Note: stamen development and pistil initiation occurred at eight WPA38. Filament elongation and ovule development occurrs at six WPA. Stamen development is nearly complete by four WPA. Data are presented as mean values ± SD. Significant differences determined by two-tailed Student’s t test indicated by asterisks **p = 0.0089, ***p = 0.00027 (n = 3); not significant, ns. d Diagram of VviPLATZ1 genomic structure, which contains five exons (boxes). Exons 3 and 4 displayed in green encode the PLATZ domain. In the f-haplotype, DNA polymorphisms altered the nucleotides at −13 bp and −5 bp in the 5′ UTR, as well as the first codon (underlined nucleotides). The arrowhead and gray highlight illustrate the adenine insertion. The start codon for the hermaphrodite (H/H) and female (f/f) VviPLATZ1 alleles is highlighted in yellow. Point mutations that alter amino acid content in exons 3, 4, and 5 are displayed by asterisk (*). The GenBank accession number for VviPLATZ1 derived from 04C023V0006 is MW548436. For the Vviplatz1 allele derived from 04C023V0003, the GenBank accession number is MW548435. Source data underlying Fig. 1c are provided as a Source Data file.
