Fig. 1: ISWI and Chd1 remodelers rely on proper nucleosome densities to generate nucleosome arrays. | Nature Communications

Fig. 1: ISWI and Chd1 remodelers rely on proper nucleosome densities to generate nucleosome arrays.

From: The biogenesis and function of nucleosome arrays

Fig. 1

a Composite plots depicting the average nucleosome organization of ~5000 genes before (WT; grey) and after histone depletion in otherwise wild-type (HD) or TKO cells (TKO HD). All strains express histones H3 and H4 from a galactose promoter. HD was induced by shifting cells from galactose-containing to glucose-containing media for 3 h, which reduced histone protein amounts by ~50% (Supplementary Fig. 1a, b). Nucleosome dyad positions were aligned to known +1 nucleosome positions of WT cells. Dashed lines in brighter color are biological replicates. b Simulated, truly random nucleosome organization downstream of a well-positioned +1 nucleosome. Nine simulations are overlayed (green). Nucleosome occupancy was fixed at 51% to simulate histone depletion conditions (see “Methods” section). TKO HD data from a is replotted for reference.

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