Fig. 2: Ependymal multicilia contain eIF components.
From: Cilia locally synthesize proteins to sustain their ultrastructure and functions
a Diagrams illustrating locations of investigated eIFs. b Ciliary localization of endogenous Eif3 components in day-10 mEPCs. Typical cilia were magnified to show details. Ac-tub labeled axonemes. c Ciliary localization of GFP-tagged Eif3 components. Cultured mEPCs were infected with lentiviral particles at day −1 to express GFP-fusion proteins and fixed at day 10 for immunostaining. d Ciliary localizations of endogenous Eif4E and Eif4G. e Ciliary localization of Eif3m and Eif4g. Isolated multicilia were imaged under three-dimensional structured illumination microscopy (3D-SIM).
