Fig. 4: PD-L1 on platelets shows functional relevance via decreasing T-cell activity. | Nature Communications

Fig. 4: PD-L1 on platelets shows functional relevance via decreasing T-cell activity.

From: Platelet PD-L1 reflects collective intratumoral PD-L1 expression and predicts immunotherapy response in non-small cell lung cancer

Fig. 4: PD-L1 on platelets shows functional relevance via decreasing T-cell activity.

a IFNγ ELISPOT assay of peptide-specific T-cells co-incubated with PD-L1+ platelets with or without anti-PD-L1 mAb pre-treatment (n = 3). b Quantification of the IFNγ ELISPOT assays (n = 3). c Flow cytometry-based quantification of indicated cytokines and surface markers for peptide stimulated CD8+ T-cells co-incubated with PD-L1+ platelets with or without anti-PD-L1 mAb pre-treatment (n = 3). d Representative FACS plots showing the gating strategy and the T-cell subpopulations after pre-sensitization, enrichment, and expansion. e Quantitative sub-phenotyping of NY-ESO-1 specific T cells using flow cytometry (n = 2). f Representative FACS plots displaying CD4+ TEM activity levels measured by INFγ expression after co-incubation with PD-L1+ platelets with or without anti-PD-L1 mAb pre-treatment (n = 3). g Quantification of INFγ+CD4+ TEM (n = 3). h IFNy fold change in CD4+ TEM (n = 3). i Representative FACS plots displaying CD4+ TEM activity levels measured by TNFα expression after co-incubation with PD-L1+ platelets with or without anti-PD-L1 mAb pre-treatment (n = 3). j Quantification of TNFα+CD4+ TEM (n = 3). k, TNFα fold change in CD4+ TEM (n = 3). b, c, g, h, j, k Data are mean ± SEM. Statistical significance was calculated by two-tailed Student’s t test. Source data are provided as a Source Data file.

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