Fig. 5: pPD-L1 correlates with T cell infiltration in NSCLC. | Nature Communications

Fig. 5: pPD-L1 correlates with T cell infiltration in NSCLC.

From: Platelet PD-L1 reflects collective intratumoral PD-L1 expression and predicts immunotherapy response in non-small cell lung cancer

Fig. 5: pPD-L1 correlates with T cell infiltration in NSCLC.

a, b Upper, Representative micrographs of NSCLC adenocarcinoma (H&E) (n = 11). Scale bar 500 µm. Lower, Immunofluorescence staining for T-cells (CD2: red, CD3: orange and PD-1: white) in the TME of a NSCLC patient presenting with low pPD-L1 (a) and high pPD-L1 (b). Each image is representative for at least two regions of interest (ROI) in each tumor sample. ROI were selected based on manual prestaining of DAPI. Scale bar 100 µm (n = 11). c–f Quantification of T cells per FoV (c), PD-1+ T cells per FoV (%) (d), infiltrating T cells per FoV (e) and PD-1+ infiltrating T cells per FoV (%) (f). pPD-L1 high vs. low was defined according to the median expression in this cohort. A total number of n = 22 ROIs (small symbols) were analyzed out of a total of n = 11 patients (large symbols). Data are mean ± SEM. Statistical significance was calculated by two-tailed Student’s t test (d–f) or two-tailed Mann–Whitney test (c). Source data are provided as a Source Data file.

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