Table 1 Overview of the different LASV L protein structures.
Identifier | Resolution [Å] | RNA ligands | PDB accession | EMDB accession | Comment |
|---|---|---|---|---|---|
APO-CORE | 3.14 | - | 7OCH | 12807 | High-resolution apo-polymerase core, best defined Zn2+ coordination site |
APO-ENDO | 3.35 | - | 7OE3 | 12860 | EN domain complete and bound to inhibitory peptide 1092–1104, well-defined interaction with polymerase core |
APO-RIBBON | 3.73 | - | 7OE7 | 12953 | α-ribbon visible |
3END-CORE | 2.70 | 3′ 1–16 | 7OEA | 12862 | Highest resolution core, 3′ RNA bound to the secondary binding site, EN bound to inhibitory peptide 1092–1104 |
3END-ENDO | 3.04 | 3′ 1–16 | 7OEB | 12863 | EN domain complete and bound to inhibitory peptide 1092–1104, 3’ RNA bound to the secondary binding site |
PRE-INITIATION | 3.34 | 5′ 0–19 3′ 1–19 | 7OJL | 12955 | dsRNA promoter bound, α-bundle and pendant visible |
MID-LINK | 3.50 | 5′ 10–19 3′ 1–19 | 7OJJ | 12861 | C terminus visible (low resolution), EN not potentially autoinhibited |
DISTAL-PROMOTER | 3.89 | 5′ 10–19 3′ 1–19 | 7OJK | 12954 | Distal dsRNA promoter bound, α-bundle and pendant visible |
ELONGATION | 2.92 | 5′ 0–19 3′ 1–19 C8 primer, UMPNPP | 7OJN | 12956 | C terminus buildable with high confidence, RNA duplex in active site, UMPNPP, EN autoinhibited by peptide 173–190 |
