Table 1 Thermodynamic and kinetic parameters describing the binding established between wt, ∆CH2 or R366E YidC and Pf3.

From: Monitoring the binding and insertion of a single transmembrane protein by an insertase

 

wt YidC 2 ms contact time

wt YidC 52 ms contact time

ΔCH2 YidC 2 ms contact time

R366E YidC 2 ms contact time

xβ (nm)

2.0 ± 1.4

0.81 ± 0.49

2.2 ± 2.0

2.3 ± 1.7

k0 (s–1)

0.03 ± 0.28

2.0 ± 9.1

0.07 ± 0.84

0.03 ± 0.31

ΔG (kBT)

15.0 ± 9.3

10.8 ± 4.5

14.2 ± 12.0

15.0 ± 10.3

  1. xβ and k0 were extracted by fitting the DFS plots with the Bell-Evans model (Fig. 5a–d). ΔG was calculated using the Arrhenius equation (Methods). Errors in xβ and k0 describe 95% CI, while errors in ΔG were calculated by propagating errors in k0.
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