Table 1 ANOVA comparisons of cleavage activity among experimental groups.

From: Exploiting a Y chromosome-linked Cas9 for sex selection and gene drive

Strain

Phenotype of F1 progeny

tRNA-gRNA (N)

SGyA (N)

Autosomal Cas9 (N)

P value

WT phenotype

100 ± 0% (5; 505)

50.5 ± 1.2% (6; 1218)

0 ± 0% (5; 907)

<0.0001

Ebony

0 ± 0% (5; 505)

0.2 ± 0.1% (6; 1218)*

0 ± 0% (5; 907)

0.1512

Forked

0 ± 0% (5; 505)

38.6 ± 1.9% (6; 1218)*

0 ± 0% (5; 907)

<0.0001

Ebony-Forked

0 ± 0% (5; 505)

10.1 ± 1.2% (6; 1218)*

0 ± 0% (5; 907)

<0.0001

Ebony-Sepia

0 ± 0% (5; 505)

0.3 ± 0.3% (6; 1218)*

4.9 ± 2.1% (5; 907)

0.0156

Ebony-Forked-Sepia

0 ± 0% (5; 505)

0.3 ± 0.3% (6; 1218)*

95.1 ± 2.1 (5; 907)

<0.0001

  1. *Recovered only males.
  2. Distinct differences between the activity of a Y-linked Cas9 and an autosomal Cas9 can be observed in the percentages of individuals with particular phenotypes. In SGyA experimental crosses, only males (with an asterisk) had mutant phenotypes. females were unaffected. The majority of F1 progeny from autosomal crosses had triple mutant phenotypes. Average percentages and SEM values are listed below. The number of replicates is listed next to the total number of F1 progeny counts. Significance was determined using a two-tailed student’s t test.
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