Fig. 2: 2A binds to a minimal 47 nt element in the viral RNA.
From: Structural and molecular basis for Cardiovirus 2A protein as a viral gene expression switch
a–b Sequences and schematic diagrams of the EMCV 1–6 constructs used to assay 2A binding. c EMSA analyses showing that removal of the 5′ extension (blue) disables 2A binding. d Microscale thermophoresis (MST) was used to quantify the interactions observed in c. All measurements were repeated as two independent experiments and error bars represent the standard deviation from the mean. RNA concentration ranges between 60 pM–20 µM (for EMCV 1) and 150 pM–5 µM (for EMCV 2–6). Source data are provided as a Source Data file.
